» Dr. Ramiro Freudenthal

Research

Synaptic dynamic of NF-kappa B during Long Term Memory Consolidation in mice.

Since the discovery that long-term memory is dependent on protein synthesis, several transcription factors have been found to participate in the transcriptional activity needed for its consolidation. Among them, NF-kappa B is a constitutive transcription factor whose nuclear activity has proven to be necessary for the consolidation of inhibitory avoidance in mice. This transcription factor has a wide distribution in the nervous system, with a well reported presence in dendrites and synaptic terminals. We report changes in synaptosomal NF-kappa B localization and activity, during long-term memory consolidation. Activity comparison of synaptosomal and nuclear NF- kappa B, indicates different dynamics for both localizations. We identified two pools of synaptosomal NF-kappa B one obtained with the synaptoplasm (free fraction) and the second bound to the synaptosomal membranes. During the early steps of consolidation the first pool is activated, as the membrane associated transcription factor fraction increases and concomitantly the free fraction decreases. These results indicate that the activation of synaptic NF-kappa B and its translocation to membranes are part of the consolidation of long term memory in mice.

Pharmacological interference of the pdz PSD-95 interactions.

Our focus is to elucidate the role of synaptic NF-kappa B during long-term memory consolidation investigating several possible interactions with other synaptic proteins such as acetyltransferases, NMDA and PSD95. We are studying this with pharmacological approach, disrupting the interaction of PSD95 and NMDA and evaluating the behavioral consequences and the effect on NF-kappa B activation.

NMDA receptor expression and membrane presentation during Long Term Memory consolidation in the crab Neohelice granulate.

Much of the work done on memory consolidation regarding NF-kappa B and NMDA was done initially with the crab species Neohelice granulata in the paradigm of contextual conditioning. This line of work continues to part of our lab were we keep exploring the interactions and dynamics of such proteins during long-term memory consolidation both in crabs and mice.

Publications

Angeles Salles, Mariano Boccia, Mariano Blake, Nicoleta Corbi, Claudio Passananti, Carlos Maria Baratti, Arturo Romano, Ramiro Freudenthal. Title: HIPPOCAMPAL DYNAMICS OF SYNAPTIC NF-kappa B DURING INHIBITORY AVOIDANCE LONG-TERM MEMORY CONSOLIDATION IN MICE. Journal: Neuroscience, Feb 2015.
Angeles Salles, Arturo Romano, Ramiro Freudenthal. Title: SYNAPTIC NF-KAPPA B PATHWAY IN NEURONAL PLASTICITY. Review Article. Journal: Journal of Physiology Paris. May, 2014.
Yanil Hepp, Martín Carbó Tano, María Eugenia Pedreira, Ramiro A. M. Freudenthal. NMDA-like receptors in the nervous system of the crab Neohelice granulata: A neuroanatomical description. Journal of Comparative Neurology. Aceptdo noviembre 2012. En prensa.. Volume 521, Issue 10, pages 2279–2297, 1 July 2013
Yang M, Carbó Tano M, Freudenthal R, Hermitte G. Characterization of the cardiac ganglion in the crab Neohelice granulata and immunohistochemical evidence of GABA-like extrinsic regulation. Arthropod Struct Dev. 2012 Sep 14. doi:pii: S1467-8039(12)00073-4. 10.1016/j.asd.2012.09.002. [Epub ahead of print].
de la Fuente V, Freudenthal R, Romano A. Reconsolidation or extinction: transcription factor switch in the determination of memory course after retrieval. J Neurosci. 2011 Apr 13;31(15):5562-73.
Fustiñana MS, Ariel P, Federman N, Freudenthal R, Romano A. Characterization of the beta amyloid precursor protein-like gene in the central nervous system of the crab Chasmagnathus. Expression during memory consolidation. BMC Neurosci. 2010 Sep 1;11:109
Boccia M*, Freudenthal R*, Blake M, de la Fuente V, Acosta G, Baratti C, Romano A. Activation of Hippocampal Nuclear Factor-{kappa}B by Retrieval Is Required for Memory Reconsolidation. J Neurosci. 2007 Dec 5; 27(49):13436-13445. * Ambos tuvieron igual participación.
Review Article: Romano, A., Freudenthal, R., Merlo, E. and Feld, M. Lessons from a crab: Molecular mechanisms in different memory phases of Chasmagnathus. Biol Bull. 2006 Jun; 210(3):280-8.
Review Article: Romano, A., Freudenthal, R., Merlo, E. and Routtenberg, A. Memories of NF-kappaB. Role of Rel/NF-kappaB transcription factor in neural plasticity and memory from crustaceans to mammals. Eur J Neurosci. 2006 Sep; 24(6):1507-16.
Ramiro Freudenthal, Mariano M. Boccia, Gabriela B. Acosta, Mariano G. Blake, Emiliano Merlo, Carlos M. Baratti and Arturo Romano.  NF-kappaB transcription factor is required for inhibitory avoidance long-term memory in mice. Eur J Neurosci. 2005 May;21(10):2845-52.
Emiliano Merlo, Ramiro Freudenthal, Héctor Maldonado and Arturo Romano. Activation of the transcription factor NF-kappaB by retrieval is required for long-term memory reconsolidation. Learn Mem. 2005 Jan-Feb;12(1):23-9.
Ramiro Freudenthal, Arturo Romano, and Aryeh Routtenberg. Transcription Factor NF-_B Activation After In Vivo Perforant Path LTP in Mouse Hippocampus. Hippocampus. 2004;14(6):677-83. Extranjera, con referato, con indexación en el Scientific Citation Index
Lia Frenkel, Ramiro Freudenthal, Arturo Romano, Hector Maldonado y Alejandro Delorenzi. Angiotensin II and transcription factor Rel/NF-kappa B link enviromental water shortage with memory improvement. Neuroscience. 2002;115(4):1079-87. Extranjera, con referato, con indexación en el Scientific Citation Index.
Emiliano Merlo, Ramiro Freudenthal y Arturo Romano, The IkB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus. Neuroscience, 2002;112(1):161-72. Extranjera, con referato, con indexación en el Scientific Citation Index.
Freudenthal, R. y Romano, A., Participation of Rel/NF-kB transcription factors in long-term memory in the crabChasmagnathus. Brain research (2000) 14; 855(2) 274-281. Extranjera, con referato, con indexación en el Scientific Citation Index.
Freudenthal, R., Locatelli, F., Hermitte, G., Maldonado, H., Lafourcade, C., Delorenzi, A. y Romano, A., Kappa-B like DNA-binding activity is enhanced after a spaced training that induces long-term memory in the crab Chasmagnathus. Neuroscience Letters (1998) 20; 242(3) 143-146. Extranjera, con referato, con indexación en el Scientific Citation Index.

 

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Group members

Dr. Ramiro Freudenthal

Dr. Ramiro Freudenthal
Investigador


Lic. Angeles Salles

Lic. Angeles Salles
Becaria Conicet